INDIGO Home University of Illinois at Urbana-Champaign logo uic building uic pavilion uic student center

Sustained delivery of vincristine inside an orthotopic mouse sarcoma model decreases tumor growth

Show full item record

Bookmark or cite this item: http://hdl.handle.net/10027/21677

Files in this item

File Description Format
PDF Harris et al JPS.pdf (826KB) Restricted to UIC (no description provided) PDF
Title: Sustained delivery of vincristine inside an orthotopic mouse sarcoma model decreases tumor growth
Author(s): Harris, J.C.; Coburn, J.M.; Kajdacsy-Balla, A.; Kaplan, D.L.; Chiu, B.
Subject(s): Sarcoma Local drug delivery Silk Vincristine
Abstract: Background Sarcoma accounts for 20% of solid tumors in children. Surgery has significant morbidity. We hypothesized that delivering chemotherapy directly into tumors through sustained release silk systems could slow tumor growth. Methods Human Ewing sarcoma cells A673 were cultured with vincristine and doxorubicin to determine half maximal inhibitory concentration (IC50). Cells were injected into mouse hind leg to create orthotopic tumors. Tumor volumes were measured using ultrasound. When volume reached > 250mm3, interventions included: implantation of drug-free silk foam (Control-F), doxorubicin 400 μg foam (Dox400-F), vincristine 50 μg foam (Vin50-F), drug-free silk gel (Control-G), vincristine 50 μg gel (Vin50-G), or single dose intravenous vincristine 50 μg (Vin50-IV). End-point was volume > 1000 mm3. Kaplan Meier and ANOVA were used. Results IC50 for vincristine and doxorubicin was 0.5 ng/mL and 200 ng/mL, respectively. There was no difference between Dox400-F [6 ± 1 days to end point (DTEP)] and Control-F (5 ± 1.3 DTEP). Vin50-F (12.4 ± 3.5 DTEP) had slower growth compared to Control-F (p < 0.001), and there was no difference between Vin50-F and Vin50-IV (14 ± 0 DTEP). Growth was slowest with Vin50-G, 28 ± 10.3 DTEP compared to all other treatment groups (p < 0.05). Conclusion Sustained delivery of vincristine inside the sarcoma tumor with silk gel decreased tumor growth. Applying this intratumoral treatment strategy may potentially decrease the extent of surgical excision.
Issue Date: 2016-12
Publisher: Elsevier
Citation Info: Harris, J. C., Coburn, J. M., Kajdacsy-Balla, A., Kaplan, D. L. and Chiu, B. Sustained delivery of vincristine inside an orthotopic mouse sarcoma model decreases tumor growth. Journal of Pediatric Surgery. 2016. 51(12): 2058-2062. DOI: 10.1016/j.jpedsurg.2016.09.040.
Type: Article
Description: NOTICE: This is the author’s version of a work that was accepted for publication in Journal of Pediatric Surgery. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Pediatric Surgery, Volume 51, Issue 12, December 2016, Pages 2058–2062. DOI: 10.1016/j.jpedsurg.2016.09.040.
URI: http://hdl.handle.net/10027/21677
ISSN: 0022-3468
Date Available in INDIGO: 2017-06-20
 

This item appears in the following Collection(s)

Show full item record

Statistics

Country Code Views
United States of America 72
China 14
Ukraine 13
Russian Federation 7
Germany 2

Browse

My Account

Information

Access Key