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Menoprogen, a TCM Herbal Formula for Menopause, Increases Endogenous E-2 in an Aged Rat Model of Menopause by Reducing Ovarian Granulosa Cell Apoptosis.

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Title: Menoprogen, a TCM Herbal Formula for Menopause, Increases Endogenous E-2 in an Aged Rat Model of Menopause by Reducing Ovarian Granulosa Cell Apoptosis.
Author(s): Li, Y; Ma, H; Lu, Y; Tan, BJ; Xu, L; Lawal, TO; Mahady, GB; Liu, D
Abstract: The effect of Menoprogen (MPG) on ovarian granulosa cell (GC) apoptosis was investigated in vitro and in vivo in an aged rat model of menopause. Intragastric administration of Menoprogen or estradiol valerate to 14-month-old senile female rats for eight weeks increased plasma E2 levels, as well as the weight of both ovarian and uterine tissues. Flow cytometric (FCM) analysis of isolated GCs from MPG-treated aged rats showed reductions in the G0/G1 ratio and apoptotic peaks. Isolated GCs also exhibited an increase in cell size and the number of cytoplastic organelles and intracellular gap junctions, the reappearance of secretory granules, and a lack of apoptotic bodies as determined by TEM. Results from a TdT-mediated dUTP nick end-labeling (TUNEL) assay revealed a reduction in TUNEL-positive GCs after MPG treatment. Immunohistochemical analysis showed a downregulation of proapoptotic Bax proteins and an upregulation of antiapoptotic Bcl-2 proteins. The addition of MPG-medicated serum to the media of cultured GCs also reduced cadmium chloride-induced apoptosis and downregulated caspase-3 protein expression. This work demonstrates that Menoprogen inhibits GC apoptosis in aged female rats and thereby increases E2 production.This represents a novel mechanism of action for this herbal medicine in the treatment of menopausal symptoms.
Issue Date: 2016
Publisher: Hindawi Publishing Corporation
Citation Info: Li, Y., Ma, H., Lu, Y., Tan, B. J., Xu, L., Lawal, T. O., Mahady, G. B. and Liu, D. Menoprogen, a TCM Herbal Formula for Menopause, Increases Endogenous E-2 in an Aged Rat Model of Menopause by Reducing Ovarian Granulosa Cell Apoptosis. Biomed Research International. 2016. DOI: 10.1155/2016/2574637.
Type: Article
Description: This is a copy of an article published in BioMed Research International. © 2015 Hindawi Publishing Corporation Publications. © 2016 Yu Li et al.
URI: http://hdl.handle.net/10027/21047
ISSN: 2314-6133
Sponsor: This study was supported by a project funded by the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions, supported by the Open Project Program of Traditional Chinese Medicine Department of Nanjing University of Chinese Medicine, Nanjing 210046, China (YS2012ZYX304), supported by the Fund of Ministry of Science Technology of China for Special Project before Major Basic Research (no. 2003CCA01700). This publication was supported in part by Grant no. R21-AT02381 from the National Center for Complementary and Integrative Health (previously NCCAM) at NIH.
Date Available in INDIGO: 2016-08-08
 

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