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A novel role for phagocytosis-like uptake in herpes simplex virus entry

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Title: A novel role for phagocytosis-like uptake in herpes simplex virus entry
Author(s): Clement, Christian; Tiwari, Vaibhav; Scanlan, Perry M; Valyi-Nagy, Tibor; Yue, Beatrice Y; Shukla, Deepak
Subject(s): Receptor-mediated endocytosis Glycoprotein-D receptor Hamster ovary cells Heparin-sulfate
Abstract: It is becoming increasingly clear that herpesviruses can exploit the endocytic pathway to infect cells, yet several important features of this process remain poorly defined. Using herpes simplex virus-1 (HSV-1) as a model, we demonstrate that endocytosis of the virions mimic many features of phagocytosis. During entry, HSV-1 virions associated with plasma membrane protrusions followed by a phagocytosis-like uptake involving rearrangement of actin cytoskeleton and trafficking of the virions in large phagosome-like vesicles. RhoA GTPase was activated during this process and the mode of entry was cell type-specific. Clathrin-coated vesicles had no detectable role in virion trafficking as Eps15 dominant-negative mutants failed to affect HSV-1 uptake. Binding and fusion of the virion envelope with the phagosomal membrane is likely facilitated by clustering of nectin-1 (or HVEM) in phagosomes, which was observed in infected cells. Collectively, our data suggests a novel mode of uptake by which the virus can infect both professional and nonprofessional phagocytes.
Issue Date: 2006-09-26
Publisher: Rockefeller University Press
Citation Info: Clement, C., Tiwari, V., Scanlan, P. M., Valyi-Nagy, T., Yue, B. Y. J. T., & Shukla, D. (2006). A novel role for phagocytosis-like uptake in herpes simplex virus entry. Journal of Cell Biology, 174, 1009-1021.
Type: Article
Description: Publishers Copyright -
ISSN: 0021-9525
Date Available in INDIGO: 2008-04-14

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