INDIGO Home University of Illinois at Urbana-Champaign logo uic building uic pavilion uic student center

Flexible ordering of antibody class switch and V(D)J joining during B-cell ontogeny

Show full item record

Bookmark or cite this item: http://hdl.handle.net/10027/20400

Files in this item

File Description Format
PDF Genes Dev.-2013-Kumar-2439-44.pdf (645KB) (no description provided) PDF
Title: Flexible ordering of antibody class switch and V(D)J joining during B-cell ontogeny
Author(s): Kumar, Satyendra; Wuerffel, Robert; Achour, Ikbel; Lajoie, Bryan; Sen, Ranjan; Dekker, Job; Feeney, Ann J; Kenter, Amy L
Subject(s): B-cell development Ag gene rearrangement gene expression
Abstract: V(D)J joining is mediated by RAG recombinase during early B-lymphocyte development in the bone marrow (BM). Activation-induced deaminase initiates isotype switching in mature B cells of secondary lymphoid structures. Previous studies questioned the strict ontological partitioning of these processes. We show that pro-B cells undergo robust switching to a subset of immunoglobulin H (IgH) isotypes. Chromatin studies reveal that in pro-B cells, the spatial organization of the Igh locus may restrict switching to this subset of isotypes. We demonstrate that in the BM, V(D)J joining and switching are interchangeably inducible, providing an explanation for the hyper-IgE phenotype of Omenn syndrome.
Issue Date: 2013-11
Publisher: Cold Spring Harbor Laboratory Press
Citation Info: Kumar, S., Wuerffel, R., Achour, I., Lajoie, B., Sen, R., Dekker, J., Feeney, A. J. and Kenter, A. L. Flexible ordering of antibody class switch and V(D)J joining during B-cell ontogeny. Genes & Development. 2013. 27(22): 2439-2444. DOI: 10.1101/gad.227165.113.
Type: Article
URI: http://hdl.handle.net/10027/20400
ISSN: 1549-5477
Sponsor: This work was supported by the National Institutes of Health (AI052400 to A.L.K., AI082918 to A.J.F., and HG003143 and HG00459 to J.D), the W.M. Keck Foundation (to J.D), and the Intramural Research Program of the National Institute on Aging (Baltimore, MD) (to R.S.).
Date Available in INDIGO: 2016-04-11
 

This item appears in the following Collection(s)

Show full item record

Statistics

Country Code Views
China 61
United States of America 34
Russian Federation 24
Ukraine 9
Germany 8

Browse

My Account

Information

Access Key