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Fractalkine receptor is expressed in mature ovarian teratomas and required for epidermal lineage differentiation

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Title: Fractalkine receptor is expressed in mature ovarian teratomas and required for epidermal lineage differentiation
Author(s): Rooper, Lisa; Gurler, Hilal; Kajdacsy-Balla, Andre A.; Barbolina, Maria V.
Subject(s): cell differentiation fractalkine receptor mature ovarian teratoma
Abstract: BACKGROUND: The goal of this study was to determine a predominant cell type expressing fractalkine receptor (CX3CR1) in mature ovarian teratomas and to establish functional significance of its expression in cell differentiation. METHODS: Specimens of ovarian teratoma and human fetal tissues were analyzed by immunohistochemistry for CX3CR1expression. Ovarian teratocarcinoma cell line PA-1 was used as a model for cell differentiation. RESULTS: We found that the majority of the specimens contained CX3CR1-positive cells of epidermal lineage. Skin keratinocytes in fetal tissues were also CX3CR1- positive. PA-1 cells with downregulated CX3CR1 failed to express a skin keratinocyte marker cytokeratin 14 when cultured on Matrigel in the presence of a morphogen, bone morphogenic protein 4 (BMP-4), as compared to those expressing scrambled shRNA. CONCLUSIONS: Here we demonstrate that CX3CR1 is expressed in both normally (fetal skin) and abnormally (ovarian teratoma) differentiated keratinocytes and is required for cell differentiation into epidermal lineage.
Issue Date: 2013-08
Publisher: BioMed Central
Citation Info: Rooper L, Gurler H, Kajdacsy-Balla AA, Barbolina MV. Fractalkine receptor is expressed in mature ovarian teratomas and required for epidermal lineage differentiation. Journal of Ovarian Research. 2013 Aug 17;6(1):57. doi: 10.1186/1757-2215-6-57
Type: Article
Description: © 2013 Rooper et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
URI: http://hdl.handle.net/10027/12837
ISSN: 1757-2215
Sponsor: American Cancer Society, Illinois Division, grant #198484 (to MVB), NIH National Cancer Institute, grant #1R21CA160917 (to MVB), and Liz Tilberis Award from the Ovarian Cancer Research Fund (to MVB).
Date Available in INDIGO: 2014-03-18
 

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