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HPLC-MTT assay: anti-cancer activity of aqueous garlic extract is from allicin

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Title: HPLC-MTT assay: anti-cancer activity of aqueous garlic extract is from allicin
Author(s): Lee, Jenny; Gupta, Shalini; Huang, Jin-Sheng; Jayathilaka, Lasanthi P.; Lee, Bao-Shiang
Subject(s): Allium sativum Garlic Aqueous garlic extract mouse colon cancer cell
Abstract: A strategy using RP-HPLC, TLC, MS, NMR, chemical synthesis, and MTT cell viability assay to identify allicin as the active anti-cancer compound in the aqueous extract of garlic (AGE) is described. Change the pH of the AGE from 7 to 5 eliminated interfering molecules and enabled a clean HPLC separation of the constituents in AGE. MTT assay of the HPLC fractions identify an active fraction. Further analysis by TLC, MS, and NMR verify the active HPLC fraction as allicin. Chemically synthesized allicin is used to provide further confirmation. The results clearly identify the active compound in AGE as allicin.
Issue Date: 2013-05
Publisher: Elsevier Masson
Citation Info: Lee, J., Gupta, S., Huang, J. S., Jayathilaka, L. P. and Lee, B. S. HPLC-MTT assay: Anticancer activity of aqueous garlic extract is from allicin. Analytical Biochemistry. 2013. 436(2): 187-189. DOI: 10.1016/j.ab.2013.01.033
Type: Article
Description: NOTICE: This is the author’s version of a work that was accepted for publication in Analytical Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Analytical Biochemistry, Vol 436, Issue 2, 2013 DOI:10.1016/j.ab.2013.01.033
URI: http://hdl.handle.net/10027/10996
ISSN: 0003-2697
Date Available in INDIGO: 2014-01-03
 

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