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Novel Device to Sample the Esophageal Microbiome — The Esophageal String Test

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Title: Novel Device to Sample the Esophageal Microbiome — The Esophageal String Test
Author(s): Fillon, Sophie A.; Harris, J. Kirk; Wagner, Brandie D.; Kelly, Caleb J.; Stevens, Mark J.; Moore, Wendy; Fang, Rui; Schroeder, Shauna; Masterson, Joanne C.; Robertson, Charles E.; Pace, Norman R.; Ackerman, Steven J.; Furuta, Glenn T.
Abstract: A growing number of studies implicate the microbiome in the pathogenesis of intestinal inflammation. Previous work has shown that adults with esophagitis related to gastroesophageal reflux disease have altered esophageal microbiota compared to those who do not have esophagitis. In these studies, sampling of the esophageal microbiome was accomplished by isolating DNA from esophageal biopsies obtained at the time of upper endoscopy. The aim of the current study was to identify the esophageal microbiome in pediatric individuals with normal esophageal mucosa using a minimally invasive, capsule-based string technology, the EnterotestTM. We used the proximal segment of the Enterotest string to sample the esophagus, and term this the ‘‘Esophageal String Test’’ (EST). We hypothesized that the less invasive EST would capture mucosal adherent bacteria present in the esophagus in a similar fashion as mucosal biopsy. EST samples and mucosal biopsies were collected from children with no esophageal inflammation (n = 15) and their microbiome composition determined by 16S rRNA gene sequencing. Microbiota from esophageal biopsies and ESTs produced nearly identical profiles of bacterial genera and were different from the bacterial contents of samples collected from the nasal and oral cavity. We conclude that the minimally invasive EST can serve as a useful device for study of the esophageal microbiome.
Issue Date: 2012-09
Publisher: Public Library of Science
Citation Info: Fillon SA, Harris JK, Wagner BD, et al. Novel Device to Sample the Esophageal Microbiome-The Esophageal String Test. Plos One. Sep 2012;7(9). DOI: 10.1371/journal.pone.0042938
Type: Article
Description: © 2012 Fillon et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI: http://hdl.handle.net/10027/10605
ISSN: 1932-6203
Sponsor: The authors recognize the support of REDCap for subjects’ database supported by the National Center for Research Resources and the National Center for Advancing Translational Sciences, National Institutes of Health, Colorado CTSI Grant Number UL1 RR025780. This work was supported by the American Partnership For Eosinophilic Disorders (APFED) Junior Faculty HOPE Research Grant (SF); National Center for Research Resources and the National Center for Advancing Translational Sciences, National Institutes of Health, Colorado CTSI Grant Number KL2 RR025779 (SF) and UL1 RR025780 (GTF) and the Campaign Urging Research for Eosinophilic Diseases (CURED) (SJA/GTF).
Date Available in INDIGO: 2013-11-22
 

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