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Characterization of Influenza Hemagglutinin Interactions with Receptor by NMR

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Title: Characterization of Influenza Hemagglutinin Interactions with Receptor by NMR
Author(s): McCullough, Christopher; Wang, Minxiu; Rong, Lijun; Caffrey, Michael
Abstract: In influenza, the envelope protein hemagglutinin (HA) plays a critical role in viral entry by first binding to sialic acid receptors on the cell surface and subsequently mediating fusion of the viral and target membranes. In this work, the receptor binding properties of influenza A HA from different subtypes (H1 A/California/04/09, H5 A/Vietnam/1205/04, H5 A/ bar-headed goose/Qinghai/1A/05, and H9 A/Hong Kong/1073/99) have been characterized by NMR spectroscopy. Using saturation transfer difference (STD) NMR, we find that all HAs bind to the receptor analogs 2,3-sialyllactose and 2,6- sialyllactose, with subtle differences in the binding mode. Using competition STD NMR, we determine the receptor preferences for the HA subtypes. We find that H5-Qinghai and H9-Hong Kong HA bind to both receptor analogs with similar affinity. On the other hand, H1 exhibits a clear preference for 2,6-sialyllactose while H5-Vietnam exhibits a clear preference for 2,3-sialyllactose. Together, these results are interpreted within the context of differences in both the amino acid sequence and structures of HA from the different subtypes in determining receptor preference.
Issue Date: 2012
Publisher: Public Library of Science
Citation Info: McCullough, C., M. Wang, et al. (2012). "Characterization of influenza hemagglutinin interactions with receptor by NMR." PLoS One 7(7): e33958.DOI: 10.1371/journal.pone.0033958
Type: Article
Description: © 2012 McCullough et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ISSN: 1932-6203
Sponsor: This work was partially supported by the University of Illinois at Chicago Center for Structural Biology.
Date Available in INDIGO: 2013-11-08

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